Meclonazepam HCl

Description

Meclonazepam HCl (5-(2-chlorophenyl)-3-methyl-7-nitro-1,3-dihydro-2H-1,4-benzodiazepin-2-one monohydrochloride) is a certified analytical reference standard belonging to the 1,4-benzodiazepine class. Structurally differentiated by a methyl substitution at the C3 stereocenter and a nitro group at position C7, this monohydrochloride salt formulation offers enhanced chemical stability and solubility for calibration and quantification workflows.

Physicochemical Properties & Identifiers:

• Systematic IUPAC Name: 5-(2-chlorophenyl)-3-methyl-7-nitro-1,3-dihydro-2H-1,4-benzodiazepin-2-one hydrochloride
• CAS Number: 58662-84-3 (free base)
• Molecular Formula: C16H12ClN3O3 · HCl
• Formula Weight: 352.20 g/mol (Free base: 315.75 g/mol)
• Physical State: Off-white to pale yellow crystalline powder
• Solubility: Soluble in DMSO (≥20 mg/mL) and methanol (≥10 mg/mL); sparingly soluble in aqueous buffers
• Melting Profile: Decomposes above 225°C

Analytical Specifications & Quality Control:

• Purity (HPLC-DAD): ≥98.0% (AUC at λ = 254 nm and 220 nm)
• Structural Identity: Verified via proton and carbon nuclear magnetic resonance (1H-NMR, 13C-NMR) and FT-IR spectroscopy
• Mass Spectrometry: Accurate mass confirmation via high-resolution ESI-MS ([M+H]+ theoretical m/z = 316.064)
• Counterion Verification: Chloride confirmed by argentometric titration / ion chromatography

Storage & Stability Guidelines:

• Store desiccated at -20°C in amber glass containers protected from light and moisture.
• Allow container to equilibrate to room temperature before opening to avoid condensation.
• Prepared working solutions in LC-MS grade methanol or acetonitrile remain stable when stored at -80°C in silanized glass vials.

Notice: This product is supplied exclusively as a technical reference standard for in vitro forensic identification, toxicology profiling, and laboratory research. It is not intended for human or animal therapeutic use.